DNA methyltransferases are required to induce heterochromatic re-replication in Arabidopsis.

ABSTRACT: The relationship between epigenetic marks on chromatin and the regulation of DNA replication is poorly understood. Mutation of the H3K27 methyltransferase genes, ARABIDOPSIS TRITHORAX-RELATED PROTEIN5 (ATXR5) and ATXR6, result in re-replication (repeated origin firing within the same cell cycle). Here we show that mutations that reduce DNA methylation act to suppress the re-replication phenotype of atxr5 atxr6 mutants. This suggests that DNA methylation, a mark enriched at the same heterochromatic regions that re-replicate in atxr5/6 mutants, is required for aberrant re-replication. In contrast, RNA sequencing analyses suggest that ATXR5/6 and DNA methylation cooperatively transcriptionally silence transposable elements (TEs). Hence our results suggest a complex relationship between ATXR5/

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Hume Stroud 

PROVIDER: E-GEOD-38286 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress