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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis of bovine oocytes with high developmental competence obtained from FSH-stimulated animals

ABSTRACT: The objective of the study was to analyze the impact of various lenght FSH withdrawal period (coasting) after the last FSH injection on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after 20, 44, 68 and 92 hours of coasting. For each cow individually (biological replicate), each coasting time was compared to others (i.e. 20 hrs vs 44 hrs; 20 hrs vs 68 hrs; 20 hrs vs 92 hrs; 44 hrs vs 68 hrs; 44 hrs vs 92 hrs and 68 hrs vs 92 hrs) for a total of six comparisons. Overall, 36 hybridizations, corresponding to the three cows and six comparisons were done using a dye-swap set-up.

ORGANISM(S): Bos taurus

SUBMITTER: Remi Labrecque

PROVIDER: E-GEOD-38345 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis of bovine oocytes with high developmental competence obtained from FSH-stimulated animals.

Labrecque Rémi R Vigneault Christian C Blondin Patrick P Sirard Marc-André MA

Molecular reproduction and development 20130424 6

Recent progress in the ovarian stimulation protocol used for bovine in vitro maturation and fertilization, especially through optimization of the follicle-stimulating hormone (FSH) withdrawal period ("coasting") after ovarian pre-treatment with FSH, has significantly improved blastocyst outcome. Despite this important success, the underlying factors leading to improved oocyte quality have not yet been identified. The aim of this project was to compare the transcriptome of germinal vesicle-stage ...[more]

PMID: 23559376

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Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes

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2016-04-01 | E-GEOD-79760 | biostudies-arrayexpress

Transcriptome analysis of FSH response in bovine cumulus cells

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Transcriptome analysis of granulosa cells after conventional vs long FSH-induced superstimulation in cattle

Project description:Conventional Superstimulation (group 1) vs. Long Superstimulation (group 3) A genome-wide bovine oligo-microarray was used to compare the gene expression of granulosa cells collected from ovarian follicles after differing durations of the growing phase induced by exogenous FSH treatment. Cows were given a conventional (4-day) or long (7-day) superstimulatory treatment (25mg FSH im at 12-h intervals; n=6 per group), followed by prostaglandin treatment with last FSH and LH treatment 24 hr later. Granulosa cells were harvested 24 h after LH treatment. The expression of 416 genes was down-regulated and 615 genes was up-regulated in the long FSH group compared to the conventional FSH group. Quantification by RT-PCR of 7 genes (NTS, PTGS2, PTX3, RGS2, INHBA, CCND2 and LRP8) followed the same trend as in microarrays. Compared to the conventional group, the long FSH group responded to exogenous LH with down-regulation of mRNA for cyclinD2, lipoprotein receptorP8, CYP51A1, CYP19A1, CJA1, INHBA, SRPINE1, and up-regulation of Vannin, POSTN, GTPAse, Cysteine. Markers of fertility and follicle maturity were up-regulated in the long FSH group. We conclude that a prolonged FSH-induced growing phase is associated with transcriptomic characteristics of greater follicular maturity and may therefore be more appropriate for optimizing the superovulatory response and developmental competence of oocytes in cattle. straight comparison of Short Superstimulation group (the reference; group 1) versus a Long Superstimulation protocol ( the treatment; group 3) using 3 different animals (biological replicates) in each group and performed dye swap. For example on array 1: group 1 cow 1 versus group 3 cow 1 (cy3 vs cy5) and on array 4 is the dye swap group 3 cow 1 versus group 1 cow 1 (cy3 vs cy5).

2016-08-01 | E-GEOD-80289 | biostudies-arrayexpress

Impact of G protein versus beta-arrestin-dependent signal transduction at the FSH receptor (affy_fsh_human)

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2011-11-25 | E-GEOD-21982 | biostudies-arrayexpress

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Chromatin maps of the Drosophila melanogaster TrxG protein Ash1 and FSH

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2013-06-30 | E-GEOD-30820 | biostudies-arrayexpress

Expression data from mouse cumulus cells

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Generation of chromatin maps for ASH1 and FSH applying ChIP-seq

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2013-02-06 | E-GEOD-36404 | biostudies-arrayexpress

The BET protein FSH functionally interacts with ASH1 to orchestrate global gene activity in Drosophila

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2013-02-06 | E-GEOD-36450 | biostudies-arrayexpress

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