Unraveling the KNOTTED1 regulatory network in maize meristems

ABSTRACT: KNOTTED1(KN1)-like homeobox (KNOX) transcription factors function in plant meristems, self-renewing structures consisting of stem cells and their immediate daughters. Despite their importance for plant development, the genomic network targeted by KNOX proteins is poorly understood. Using ChIP-seq, we defined the KN1 cistrome in maize inflorescences and found that KN1 binds to several thousand loci. To understand how these binding occupancies correlate with changes in transcriptional regulation, we performed RNA-seq on immature ears and tassels, and compared expression profiles between normal and loss-of-function kn1 plants, in addition to immature leaves from normal and gain-of-function Kn1 plants. We found that 643 of the KN1 targets were modulated in one or multiple tissues, with a stron

ORGANISM(S): Zea mays

SUBMITTER: Maria Mejia Guerra 

PROVIDER: E-GEOD-38487 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress