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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide maps of H3K27ac localization in HUVECs

ABSTRACT: H3K27Ac is one of the expressed enhancer markers in endothelial cells, but its genomic localization is unknown. This time, we established a new antibody for H3K27ac, and performed ChIP-seq to identify H3K27ac binding site in whole genome manner under hypoxia. We used chromatin immunoprecipitation with deep sequencing (ChIP-seq) of HUVECs treated with or without hypoxia (1%O2) for 24hours, then H3K27ac binding regions were identified. Normoxia was used as a control condition. HUVECs were used within the first 6 passages.

ORGANISM(S): Homo sapiens

SUBMITTER: Takahide Kohro

PROVIDER: E-GEOD-38555 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide maps of H3K27ac, PPARβ/δ and Pol II localization in HUVECs

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