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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional response to tetanus vaccination in porcine PBMCs

ABSTRACT: Three groups of German Landrace piglets were vaccinated with tetanus toxoid. Transcriptome profiles of PBMC were analysed from blood samples taken 0, 2, 4, 8, 24 and 75 hours after a first vaccination and 0, 2, 4, 8, 24 and 75 hours as well as 14 d after a second vaccination on day 14. pair wise comparison of 0 hours to the respective time points after each vaccination

ORGANISM(S): Sus scrofa

SUBMITTER: Klaus Wimmers

PROVIDER: E-GEOD-38602 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic response of porcine PBMCs to vaccination with tetanus toxoid as a model antigen.

Adler Marcel M Murani Eduard E Brunner Ronald R Ponsuksili Siriluck S Wimmers Klaus K

PloS one 20130325 3

The aim of the present study was to characterize in vivo genome-wide transcriptional responses to immune stimulation in order to get insight into the resulting changes of allocation of resources. Vaccination with tetanus toxoid was used as a model for a mixed Th1 and Th2 immune response in pig. Expression profiles of PBMCs (peripheral blood mononuclear cells) before and at 12 time points over a period of four weeks after initial and booster vaccination at day 14 were studied by use of Affymetrix ...[more]

PMID: 23536793

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Transcriptional response to tetanus vaccination in porcine PBMCs

Project description:Three groups of German Landrace piglets were vaccinated with tetanus toxoid. Transcriptome profiles of PBMC were analysed from blood samples taken 0, 2, 4, 8, 24 and 75 hours after a first vaccination and 0, 2, 4, 8, 24 and 75 hours as well as 14 d after a second vaccination on day 14.

2012-12-31 | GSE38602 | GEO

Proteomic identification of anti-tetanus toxoid antibodies from human serum (HD1)

Project description:Proteomic identification and characterization of antibodies comprising the serological response to antigen can provide unique insight into the functional dynamics of adaptive immunity. We have developed a novel method to overcome the technical challenges which previously limited the direct analysis of immunoglobulin proteins in serum, as demonstrated by the identification of human anti-tetanus toxoid (TT) immunoglobulin G (IgG) proteins following booster vaccination. We analyzed the serum IgG repertoire across four time-points corresponding to pre-vaccination, 7 days, 3 months, and 9 months post vaccination. Antigen-specific antibodies were affinity purified against immobilized TT protein and sequenced by bottom-up nanoLC-MS/MS. Interpretation of mass spectra required a custom reference database of IgG heavy and light chain variable sequences determined by NextGen RNA sequencing of the donor's circulating plasmablasts and memory B cells following booster vaccination.

2014-04-24 | PXD000917 | Pride

Transcriptional response to tetanus toxoid in pigs with divergent humoral and lean growth performance

Project description:In vivo microarray study of transcriptional changes in porcine peripheral blood mononuclear cells (PBMCs) during the immune response to tetanus vaccination Comparisons of high (hiLG) vs. low lean growth (loLG) and high antibody titer (hiAB) vs. low AB (loAB) Expt type: Mixed model including repeated measures

2013-12-31 | E-GEOD-47845 | biostudies-arrayexpress

Proteomics of Bordetella pertussis whole-cell and acellular vaccines

Project description:Bordetella pertussis is the etiological agent of whooping cough, a bacterial infection of especially children, which may be fatal without treatment. In frame of studies to investigate putative effects of vaccination on host-pathogen interaction and clonal distribution of strains, in addition to Corynebacterium diphtheriae and Clostridium tetani toxoid vaccines, also whole-cell and acellular pertussis vaccines were analyzed by mass spectrometry.

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Functional heterogeneity of human memory T cell clones primed by pathogens or vaccines

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Transcriptional response to tetanus toxoid in pigs with divergent humoral and lean growth performance

Project description:In vivo microarray study of transcriptional changes in porcine peripheral blood mononuclear cells (PBMCs) during the immune response to tetanus vaccination

2013-12-31 | GSE47845 | GEO

Vaccine Therapy and Chemotherapy With or Without Tetanus Toxoid Compared With Chemotherapy Alone in Treating Patients With Metastatic Colorectal Cancer

Project description:RATIONALE: Vaccines may make the body build an immune response to kill tumor cells. Drugs used in chemotherapy use different ways to stop tumor cells from dividing so they stop growing or die. Tetanus toxoid may make tumor cells more sensitive to chemotherapy and vaccine therapy. PURPOSE: Randomized phase II trial to study the effectiveness of chemotherapy and vaccine therapy with or without tetanus toxoid compared with chemotherapy alone in treating patients who have metastatic colorectal cancer.

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Transcriptional response to tetanus toxoid in pigs with divergent temperament (High-Reactive vs. Low-Reactive) [day 28]

Project description:in vivo microarray study of transcriptional changes in porcine peripheral blood mononuclear cells (PBMCs) during the immune response to tetanus vaccination.

2014-12-01 | GSE55415 | GEO

Transcriptional response to tetanus toxoid in pigs with divergent temperament (High-Reactive vs. Low-Reactive) [day 14]

Project description:in vivo microarray study of transcriptional changes in porcine peripheral blood mononuclear cells (PBMCs) during the immune response to tetanus vaccination.

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