Transcription profiling of LSK cells from a mouse model where expression of HOXA10 is tightly regulated in a graded, doxycyclin-dependent manner reveals that several key commitment steps in hematopoietic differentiation have distinctly different outcomes depending on the expression level of HOXA10

ABSTRACT: The homeobox (Hox) transcription factor HOXA10 has been implicated in regulation of hematopoietic cell fate. Here, using a transgenic mouse model where expression of HOXA10 is tightly regulated in a graded, doxycyclin-dependent manner we demonstrate that several key commitment steps in hematopoietic differentiation have distinctly different outcomes depending on the expression level of HOXA10. Similarly, HOXA10 regulates hematopoietic stem cell (HSC) proliferation in a dose dependent manner, since intermediate levels of HOXA10 generated a 4.5-fold increase in long-term repopulating capacity after 13 days of liquid culture, whereas high levels reduced proliferation of HSCs. Interestingly, the effects on HSC proliferation were associated with altered expression of several known regulators of

ORGANISM(S): Mus musculus

SUBMITTER: Mattias Magnusson 

PROVIDER: E-GEOD-3861 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress