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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling of a genome-reduced strain in Schizosaccharomyces pombe

ABSTRACT: Gene number of the fission yeast Schizosaccharomyces pombe is the lowest in free-living eukaryotes. We further reduced the gene number to examine a minimal gene set required for growth. The genome-reduced strain, named ASP3894, has four chromosomal deletion regions: 168.4 kb deletion in the left arm of chromosome 1 (ALT), 155.4 kb deletion in the right arm of chromosome 1 (ART), 211.7 kb deletion in the left arm of chromosome 2 (BLT) and 121.6 kb deletion in the right arm of chromosome 2 (BRT). As a result of the 657.3 kb genome reduction, 224 genes of approximately 5100 total genes were reduced. ASP3894 transcriptional profiling was compared with a parent strain, ASP3880, that is a non-genome-reduced strain to examine the influence of genome reduction. Three independent experiments. 3 control ASP3880 samples and 3 genome-reduced strain ASP3894 samples were independently grown in EMM medium and harvested at the logarithmic phase.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Satoshi Kondo

PROVIDER: E-GEOD-38620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterization of genome-reduced fission yeast strains.

Sasaki Mayumi M Kumagai Hiromichi H Takegawa Kaoru K Tohda Hideki H

Nucleic acids research 20130405 10

The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the left arm of chromosome I, 155.4 kb in the right arm of chromosome I, 211.7 kb in the left arm of chromosome II and 121.6 kb in the right arm of chromosome II. The deletions corresponde ...[more]

PMID: 23563150

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Transcriptional profiling of a genome-reduced strain in Schizosaccharomyces pombe

Project description:Gene number of the fission yeast Schizosaccharomyces pombe is the lowest in free-living eukaryotes. We further reduced the gene number to examine a minimal gene set required for growth. The genome-reduced strain, named ASP3894, has four chromosomal deletion regions: 168.4 kb deletion in the left arm of chromosome 1 (ALT), 155.4 kb deletion in the right arm of chromosome 1 (ART), 211.7 kb deletion in the left arm of chromosome 2 (BLT) and 121.6 kb deletion in the right arm of chromosome 2 (BRT). As a result of the 657.3 kb genome reduction, 224 genes of approximately 5100 total genes were reduced. ASP3894 transcriptional profiling was compared with a parent strain, ASP3880, that is a non-genome-reduced strain to examine the influence of genome reduction.

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