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The ERRalpha metabolic nuclear receptor controls growth of colon cancer cells

ABSTRACT: Estrogen-Related Receptor alpha (ERRÎ±) is a nuclear receptor that acts principally as a regulator of metabolism processes particularly in tissues subjected to high-energy demand. Besides its implication in energy metabolism and mitochondrial biogenesis, ERRÎ± was recently associated with tumorigenesis. Notably, increased expression of ERRÎ± was noted in different cancerous tissues as breast, ovary and colon. However, supplemental studies are required to better understand the role of ERRÎ± in colon carcinoma. We used microarrays to detail gene expression during ERRalpha knockdown in HCT116 cells and identified distinct classes of up and down-regulated genes. Triplicate of HCT116 cell line was infected witn shscrambled(shCTL) and shERRalpha lentivirus and used for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Julie Carrier

PROVIDER: E-GEOD-38690 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The ERRalpha metabolic nuclear receptor controls growth of colon cancer cells

Project description:Estrogen-Related Receptor alpha (ERRα) is a nuclear receptor that acts principally as a regulator of metabolism processes particularly in tissues subjected to high-energy demand. Besides its implication in energy metabolism and mitochondrial biogenesis, ERRα was recently associated with tumorigenesis. Notably, increased expression of ERRα was noted in different cancerous tissues as breast, ovary and colon. However, supplemental studies are required to better understand the role of ERRα in colon carcinoma. We used microarrays to detail gene expression during ERRalpha knockdown in HCT116 cells and identified distinct classes of up and down-regulated genes.

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