Modeling tumor subtypes in vivo using lineage restricted transgenic shRNA

ABSTRACT: Expression analysis from two genetically engineered mouse models of osteosarcoma determine the expression profile of mouse osteosarcoma Human osteosarcoma (OS) is comprised of three different subtypes: fibroblastic, chondroblastic and osteoblastic. We previously generated a mouse model of fibroblastic OS by conditional deletion of p53 and Rb in osteoblasts. Here we report an accurate mouse model of the osteoblastic subtype using shRNA-based suppression of p53. Like human OS, tumors frequently present in the long bones and preferentially disseminate to the lungs; features less consistently modeled using Cre:lox approaches. Our approach allowed direct comparison of the in vivo consequences of targeting the same genetic drivers using different technology. This demonstrated that the effects of

ORGANISM(S): Mus musculus

SUBMITTER: Carl Walkley 

PROVIDER: E-GEOD-38742 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress