Identification of DNA-replication domains in single S-phase cells: part 1 whole genome amplified single-cell DNA

ABSTRACT: DNA-replication is a key process in life and can lead to disease when disturbed. Cell-type specific early and late replication domains have been discovered throughout genomes by analysis of DNA from populations of cells. However, cell to cell differences and the association of these differences with other cellular processes remain largely elusive. Here we demonstrate for the first time that consecutive domains of early and late DNA-replication can be detected in single S-phase cells using array comparative genomic hybridization, providing proof-of-concept for a novel tool to investigate DNA-replication genome wide at the single-cell level. Furthermore, methods to profile the genome of a single cell for DNA-copy number aberrations are revolutionizing both basic genome research and clinical

ORGANISM(S): Homo sapiens

SUBMITTER: Niels Van der Aa 

PROVIDER: E-GEOD-38758 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress