Bubble-seq Analysis of the Human Genome Reveals Distinct Chromatin-Mediated Mechanisms for Regulating Early- and Late-Firing Origins

ABSTRACT: We have devised a method for isolating virtually pure and comprehensive libraries of restriction fragments that contained replication initiation sites (bubbles) in vivo (Mesner et al. 2009). We have now sequenced and mapped the bubble-containing fragments from GM06990, a near-normal EBV-transformed lymphoblastoid cell line, and have compared origin distributions to a comprehensive replication timing study recently published for this cell line. We find that early-firing origins overwhelmingly represent zones, associate only marginally with active transcription units, are localized within large domains of open chromatin, and are significantly associated with DNAseI hypersensitivity. Origin density falls from early- to mid-S-phase, but, surprisingly, rises again in late-S-phase to density le

ORGANISM(S): Homo sapiens

SUBMITTER: Veena Valsakumar 

PROVIDER: E-GEOD-38809 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress