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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of ORB1 target genes using inducible overexpression system

ABSTRACT: The aim was to identify genes associated to the down-regulation TARGET OF RAPAMYCIN (TOR) pathway in Arabidopsis. For this purpose, three independent amiR-tor lines (amiR-tor9, amiR-tor17 and amiR-tor 20) and EV lines 3 and 6 days after EST- or non-induction were used for expression profile analysis using Affymetrix microarray. Two weeks-old transgenic seedlings were transferred to MS plate with 20ÂµM estradiol to induce the overexpression of amiR-tor (consequently, repression of AtTOR levels) under the control of an estradiol-(EST) inducible promoter. Identically treated wild-type Col-0 and pER8 empty-vector (EV) transformed seedlings were served as controls. Seedlings were harvested after 3 and 6 (amiR-tor lines) days. genetic modification

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Camila Caldana

PROVIDER: E-GEOD-38878 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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