Comprehensive expression analysis of primary hepatocytes under four different culture conditions

ABSTRACT: Primary hepatocytes have been widely explored as cell sources for the study of in vitro drug metabolism and pharmacokinetics (DMPK). Aiming toward establishing an in vitro drug screening method, the current study illustrated a comprehensive increase in the DMPK-related gene expression of nanopillar (NP)-cultured 3D-spheroid. To examine the expressional changes in DMPK-related genes under four different conditions, namely, NP-, sandwich (SW)-, monolayer (ML)-cultured rat hepatocytes, and freshly isolated hepatocytes, genome-wide gene-expression analysis using a DNA microarray was performed. Among the DMPK-related genes, cytochrome P450, UDP-glucuronosyltransferase, and transporter genes were focused on. Principal component analysis showed that the global gene expression profile in sample fr

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ryosuke Takahashi 

PROVIDER: E-GEOD-38950 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress