Human bone marrow mesenchymal stem cells, young vs. old donors, early vs. late passages

ABSTRACT: Mesenchymal stem/stromal cells (MSCs) with immunosuppressive properties are increasingly used in advanced cellular therapies. Since the clinical use of hMSCs demands sequential cell expansions, we studied the effect of cell doublings on the phospholipid profile as well as functionality of human bone marrow mesenchymal stem cells (hBMSCs). In addition to the structural role of phospholipids in cell membranes, they provide precursors for eicosanoids and other signalling lipids modulating cellular functions. The hBMSCs, harvested from young adult and old donors (n=5 for both), showed clear compositional changes during cultivation, seen at the level of lipid classes, lipid species and acyl chains. As the main finding at the lipid class level, the ratio of phosphatidylinositol to phosphatidylse

ORGANISM(S): Homo sapiens

SUBMITTER: Dario Greco 

PROVIDER: E-GEOD-39035 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress