Gamma irradiation does not induce detectable changes in DNA methylation in exposed human cells

ABSTRACT: Ionizing radiation, released during accidents at nuclear power plants or after atomic bomb explosions, is a potentially serious health threat for the exposed human population. This type of high-energy radiation causes DNA damage including single- and double-strand breaks and induces chromosomal rearrangements and mutations, but it is not known if ionizing radiation directly induces changes in the epigenome of irradiated cells. We treated normal human fibroblasts and normal human bronchial epithelial cells with different doses of gamma-radiation emitted from a cesium 137 (137Cs) radiation source. After a recovery period, we analyzed global DNA methylation patterns in the irradiated and control cells using the methylated-CpG island recovery assay (MIRA) method in combination with high-resolu

ORGANISM(S): Homo sapiens

SUBMITTER: Xiwei Wu 

PROVIDER: E-GEOD-39038 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress