Cohesin association to replication sites depends on Rad50 and promotes fork restart

ABSTRACT: The cohesin complex holds together newly-replicated chromatids and is involved in diverse pathways that preserve genome integrity. We show that in budding yeast, cohesin is transiently recruited to active replication origins and it spreads along DNA as forks progress. When DNA synthesis is impeded, cohesin accumulates at replication sites and is critical for the recovery of stalled forks. Cohesin enrichment at replication forks does not depend on H2A(X) formation, which differs from its loading requirements at DNA double-strand breaks (DSBs). However, cohesin localization is largely reduced in rad50delta mutants and cells lacking both Mec1 and Tel1 checkpoint kinases. Interestingly, cohesin loading at replication sites depends on the structural features of Rad50 that are important for brid

ORGANISM(S): Saccharomyces cerevisiae W303

SUBMITTER: Philippe Pasero 

PROVIDER: E-GEOD-39078 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress