Senataxin associates with replication forks to protect fork integrity across RNAPII transcribed genes

ABSTRACT: Here we analysed the role of yeast Senataxin (Sen1) in coordinating replication with transcription and in protecting genome integrity. Senataxin is mutated in the two severe neurodegenerative diseases AOA2 and ALS4. We show that a fraction of Sen1/Senataxin DNA/RNA helicase associates with replication forks and protects the integrity of those fork encountering highly expressed RNAPII genes. sen1 mutants accumulate aberrant DNA structures and RNA-DNA hybrids while forks clash head-on with RNAPII transcription units and counteract recombinogenic events and accumulation of checkpoint signals. Nrd1, which acts togheter with Sen1 in trascription temination, is not recruited at replication forks. nrd1 mutants does not display replication defects, high genome instability and checkpoint activation

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Rodrigo Bermejo 

PROVIDER: E-GEOD-39139 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress