Increased levels of aneuploidy and deregulation of ploidy controlling genes are associated with maintained cultures of hMSC

ABSTRACT: Human mesenchymal stem cells (hMSC) are being successfully evaluated for the treatment of a wide range of pathological conditions, including graft versus host disease (GVHD), bone and cartilage degeneration, complex fistula and myocardial infarction. Many of these clinical trials use hMSC, which have been previously expanded in vitro for 8-12 weeks under pro-oxidative M-bM-^@M-^\standardM-bM-^@M-^] cell culture conditions. These conditions could have negative effects over genetic stability and promote mutations and chromosomal abnormalities. Our FISH (Fluorescence in situ hybridization) analysis shows that aneuploidy is not unusual phenomenon in conventional cultures of hMSC and that it progressively increases with the passages. We further demonstrate that senescence is linked to transcrip

ORGANISM(S): Homo sapiens

SUBMITTER: Juan Estrada 

PROVIDER: E-GEOD-39250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress