Genomic dissection of small RNAs in wild rice (O.rufipogon): lessons for rice domestication [degradome]

ABSTRACT: The lack of MIRNA set and genome sequence of O. rufipogon (the ancestor of the cultivated rice) has limited to answer the role of MIRNA genes in rice domestication.In this study, a genome, three small RNA populations and a degradome of O.rufipogon were sequenced by Illumina platform and miRNA expression were investigated by miRNA chips. A de novo genome was assembled using ~55x coverage of raw sequencing data and a total of 387 MIRNAs were identified in the O. rufipogon genome based on ~5.2 million unique small RNA reads from three different tissues of O. rufipogon. Of these O. rufipogon MIRNAs, 259 were not found in the cultivated rice, suggesting loss of these MIRNAs in the cultivated rice. We also found that 48 MIRNAs were novel in the cultivated rice, suggesting that they were potentia

ORGANISM(S): Oryza rufipogon

SUBMITTER: Yan chenghai 

PROVIDER: E-GEOD-39307 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress