Gene expression profiling of the effects of Cyclophosphamide on peripheral blood mononuclear cells

ABSTRACT: Certain anticancer drugs, particularly cyclophosphamide (CTX), were shown to enhance the antitumor efficacy of immunotherapy through different immunomodulatory mechanisms. A better understanding of the cellular and molecular basis of CTX-mediated immunomodulation is needed to improve the efficacy of chemoimmunotherapy. Transcript profiling and flow cytometry were used to explore CTX immune-enhancing activity in patients with hematologic malignancies. A single high-dose treatment quickly (1-2 days) induced an extensive transcriptional modulation in peripheral blood mononuclear cells, leading to the reduction of cell cycle and biosynthetic/metabolic processes and to the augmentation of transcripts related to DNA damage and cell death (p53 signaling pathway, CDKN1A, CCND3, BAX, BBC3, BID, DDB

ORGANISM(S): Homo sapiens

SUBMITTER: Federica Moschella 

PROVIDER: E-GEOD-39324 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress