Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Sporadic and Post-Chernobyl Papillary Thyroid Cancers

ABSTRACT: We compared the expression profiles of papillary thyroid tumors from the Chernobyl Tissues Bank (CTB) with tumors from French patients with no history of exposure to radiations. Keywords: papillary thyroid cancer vs. patient-matched healthy adjacent thyroid thyroid papillary cancer vs. patient-matched adjacent nontumor thyroid tissues. -14 tumors from France -12 tumors from Ukraine

ORGANISM(S): Homo sapiens

SUBMITTER: Vincent Detours

PROVIDER: E-GEOD-3950 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression and the biological phenotype of papillary thyroid carcinomas.

Delys L L Detours V V Franc B B Thomas G G Bogdanova T T Tronko M M Libert F F Dumont J E JE Maenhaut C C

Oncogene 20070709 57

The purpose of this paper is to correlate the molecular phenotype of papillary thyroid carcinoma (PTC) to their biological pathology. We hybridized 26 PTC on microarrays and showed that nearly 44% of the transcriptome was regulated in these tumors. We then combined our data set with two published PTC microarray studies to produce a platform- and study-independent list of PTC-associated genes. We further confirmed the mRNA regulation of 15 genes from this list by quantitative reverse transcriptio ...[more]

PMID: 17621275

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Project description:Background: For thyroid tumorigenesis, two main human in vitro models are available: primary cultures of human thyrocytes treated with TSH or EGF/serum as models for autonomous adenomas (AA) or papillary thyroid carcinomas (PTC) respectively, and human thyroid tumor derived cell lines. Previous works of our group have assessed properties of those models, with a special emphasis on mRNA regulations. It is often assumed that miRNA may be one of the primary events inducing these mRNA regulations. Methods: The purpose of this study was to investigate the representativity of those models to study microRNA regulations and their relation with mRNA expression. To achieve this aim, the miRNA expressions profiles of primary cultures treated with TSH or EGF/serum and of 6 thyroid cancer cell lines were compared to the expression profiles of 35 tumor tissues obtained by microarrays. Results: Our data on primary cultures have shown that the TSH or EGF/serum treatment did not greatly modify the microRNA expression profiles, which is contrary to what is observed for mRNA expression profiles, although they still evolved differently according to the treatment. The analysis of miRNA and mRNA expressions profiles in the cell lines has shown that they have evolved into a common, dedifferentiated phenotype, closer to ATC than to the tumors they are derived from. Conclusions: Long-terms TSH or EGF/serum treatments do not mimic AA or PTC respectively in terms of miRNA expression as they do for mRNA, suggesting that the regulations of mRNA expression induced by these physiological agents occur independently of miRNA. The general patterns of miRNA expression in the cell lines suggest that they represent a useful model for undifferentiated thyroid cancer. Mirna probably do not mediate the rapid changes in gene expression in rapid cell biology regulation. miRNA expressions profiles of primary cultures treated with TSH or EGF/serum and of 6 thyroid cancer cell lines were compared to the expression profiles of 35 tumor tissues obtained by microarrays. Please note that there are two raw data files per sample (indicated in the description field) and sample data table contains data combined across arrays (described in the sample data processing field). The raw data file 1 is labelled as 'tumor-Hy5+normal tissue-Hy3' and the raw data file 2 is labelled as tumor-Hy3+normal tissue-Hy5 as dye-swap experiments.

Dataset Information

Sporadic and Post-Chernobyl Papillary Thyroid Cancers

Publications

Gene expression and the biological phenotype of papillary thyroid carcinomas.

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