Genome wide profiling of RNA polymerase III in Saccharomyces cerevisiae

ABSTRACT: Genome wide mapping of RNA polymearase III binding sites in Saccharomyces cerevisiae under normal growth and nutrient starved condition using ChIP-seq. Chromatin Immuno-precipitation (ChIP) was performed for FLAG tagged version of pol III subunit RPC128 after crosslinking the log-phase cells with formaldehyde. MOCK and IP DNA was sequenced and coverage of pol III was calculated at each base of the genome. RPC128-FLAG ChIP-seq single end seqquencing on Illumina GAII. 2 replicates of IP samples and 1 MOCK sample. Done in under normal growth and nutrient deprivation (4 hours).

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Yatendra Kumar 

PROVIDER: E-GEOD-39566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress