Braveheart is a long non-coding RNA necessary for cardiac lineage commitment

ABSTRACT: Purpose: Long non-coding RNAs (lncRNAs) display development-specific gene expression patterns, yet we know little about their precise roles in lineage commitment. Here, we discover a novel mammalian heart-associated lncRNA, AK143260, necessary for cardiac lineage specification. Methods: Gene expression profiles of mouse ESCs and differentiated organs were analyzed for master regulators of lineage commitment. The AK143260 transcript was shown to be strongly expressed in mESCs and in cells undergoing cardiac differentiation. Its role in cardiac differentiation was examined using depletion and in vitro differentiation systems, with morphological and gene expression profiling at different time-points. Results: mESCs depleted of AK143260, named Braveheart, fail to differentiate into cardiomyocy

ORGANISM(S): Mus musculus

SUBMITTER: Laurie Boyer 

PROVIDER: E-GEOD-39656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress