Global identification and characterization of intermediate-size non-coding RNAs in Oryza sativa

ABSTRACT: In this study, we employed a special size fractionation and cDNA library construction method followed by 454 deep sequencing to systematically profile rice intermediate-size ncRNAs. Our analysis resulted in the identification of 1349 ncRNAs in total, including 754 novel ncRNAs of an unknown functional category. Chromosome distribution of all identified ncRNAs showed no strand bias, and displayed a pattern similar to that observed in protein-coding genes with few chromosome dependencies. More than half of the ncRNAs were centered around the plus-strand of the 5’ and 3’ termini of the coding regions. The majority of the novel ncRNAs were rice specific, while 78% of the small nucleolar RNAs (snoRNAs) were conserved. Tandem duplication drove the expansion of over half of the snoRNA gene famili

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Wei Chen 

PROVIDER: E-GEOD-39695 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress