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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The Wilms' tumour 1 transcription factor regulates epigenetic states via DNA methyltransferase 3A

ABSTRACT: The Wilms' tumour 1 transcription factor regulates epigenetic states via DNA methyltransferase 3A. The data consists of two Nimblegen promoter/CpG island microarrays hybridized with MCIP DNA immunoprecipitated from either a HEK293-derived cell line expressing a heterologous WT1 cDNA (W210) or a control cell line containing the expression vector (IP100), both hybridized against input DNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Anthony Dallosso

PROVIDER: E-GEOD-39713 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Control of epigenetic states by WT1 via regulation of de novo DNA methyltransferase 3A.

Szemes Marianna M Dallosso Anthony R AR Melegh Zsombor Z Curry Thomas T Li Yifan Y Rivers Caroline C Uney James J Mägdefrau Ann-Sophie AS Schwiderski Karolin K Park Ji H JH Brown Keith W KW Shandilya Jayasha J Roberts Stefan G E SG Malik Karim K

Human molecular genetics 20121005 1

Although tumour suppressor gene hypermethylation is a universal feature of cancer cells, little is known about the necessary molecular triggers. Here, we show that Wilms' tumour 1 (WT1), a developmental master regulator that can also act as a tumour suppressor or oncoprotein, transcriptionally regulates the de novo DNA methyltransferase 3A (DNMT3A) and that cellular WT1 levels can influence DNA methylation of gene promoters genome-wide. Specifically, we demonstrate that depletion of WT1 by short ...[more]

PMID: 23042785

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