Comparative Transcript Abundance in E. Coli Degradosome Mutants and their Parental Strains

ABSTRACT: Comparative transcript abundance in E. Coli degradosome mutants and their parental strains. Abstract: RNase E, an essential endoribonuclease of Escherichia coli, interacts through its C-terminal region with multiple other proteins to form a complex termed the RNA degradosome. To investigate the degradosome's proposed role as an RNA decay machine, we used DNA microarrays to globally assess alterations in the steady-state abundance and decay of 4,289 E. coli mRNAs at single-gene resolution in bacteria carrying mutations in the degradosome constituents RNase E, polynucleotide phosphorylase, RhlB helicase, and enolase. Our results show that the functions of all four of these proteins are necessary for normal mRNA turnover. We identified specific transcripts and functionally distinguishable tra

ORGANISM(S): Escherichia coli

SUBMITTER: Sue Lin-Chao 

PROVIDER: E-GEOD-3977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress