Gene expression changes in Dhcr7 knockout brain, lung and liver at E18.5 compared to wild-type embryos

ABSTRACT: The genetic defect underlying the human Smith-Lemli-Opitz dysmorphological disorder is loss-of-function mutations affecting the cholesterol synthesis enzyme dehydrocholesterol delta7 reductase, DHCR7. Dhcr7 knockout mice recapitulate the biochemical characteristics, but all knockout pups die within 14h of birth. Tissues of knockout mice accumulate the precursor sterol, 7-dehydrocholesterol, and show reduced levels of cholesterol (J. Clin. Invest. (2001) 108: 905-915). We compared the global gene expression changes in lung, liver and brain from knockout mice to those seen from organs harvested from same-pregnancy wild-type embryos, harvested before birth (E18.5). Since the P0 knockout pups die, we expected that there would be significant changes in gene expression between knockout and wild-

ORGANISM(S): Mus musculus

SUBMITTER: Michael Falduto 

PROVIDER: E-GEOD-39811 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress