Expression data from mouse embryonic stem cells and ES cell-derived definitive endoderm and early hepatic cells

ABSTRACT: Foxa2 is required for endoderm differentiation into the hepatic lineage. The mechanism of activation for Foxa2 during this developmental process has not been elucidated yet. We established an in vitro system to guide ES cells differentiating into definitive endoderm (DE) cells and the following DE cells to early hepatic cells. ChIP-seq assays have been successfully performed to assess the Foxa2 binding profile. Over 50% of Foxa2 target genes in DE cells were found to be activated in hepatic cells which were at a stage later than the DE stage. Therefore, our finding at the genome-wide level proved Foxa2 serves as a pioneer factor at the DE stage. Furthermore, Foxa2 could specifically induce H3K4me2 modifications to the promoter/enhancer regions of many hepatic genes to pre-mark the chromati

ORGANISM(S): Mus musculus

SUBMITTER: Chenhuan Xu 

PROVIDER: E-GEOD-39854 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress