LIN28 binds messenger RNAs at GGAGA motifs and regulates splicing factor abundance (splicing array)

ABSTRACT: LIN28 is a conserved RNA binding protein implicated in pluripotency, reprogramming and oncogenesis. Previously shown to act primarily by blocking let-7 microRNA (miRNA) biogenesis, here we elucidate distinct roles of LIN28 regulation via its direct messenger RNA (mRNA) targets. Through cross-linking and immunoprecipitation coupled with high-throughput sequencing (CLIP-seq) in human embryonic stem cells and somatic cells expressing exogenous LIN28, we have defined discrete LIN28 binding sites in a quarter of human transcripts. These sites revealed that LIN28 binds to GGAGA sequences enriched within loop structures in mRNAs, reminiscent of its interaction with let-7 miRNA precursors. Among LIN28 mRNA targets, we found evidence for LIN28 autoregulation and also direct but differing effects on

ORGANISM(S): Homo sapiens

SUBMITTER: Gene Yeo 

PROVIDER: E-GEOD-39855 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress