Spp1, a member of Set1 complex, promotes meiotic DSB formation by tethering histone H3K4 methylation sites to chromosome axes

ABSTRACT: Meiotic DSB, catalyzed by the Spo11 transesterase protein and accessory DSB proteins, form in the nucleosome depleted regions (NDR) at promoters, preferentially those located on the chromosome loops that shape meiotic chromosomes, whereas the DSB proteins are located on chromosome axes at the basis of these loops. Mechanisms bridging these two chromosomal regions for DSB formation have remained elusive. Here we show that Spp1, a conserved member of the histone H3K4 methyltransferase Set1 complex, is required for normal levels of DSB formation and is associated with chromosome axes in the DSB-rich domains during meiosis. Moreover, Spp1 physically interacts with the Mer2 axis-associated DSB protein, and uses its PHD finger as a magnet to read H3K4 trimethylation close to promoters, tether th

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Valerie Borde 

PROVIDER: E-GEOD-39900 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress