Transcriptome-wide Regulation of Splicing and mRNA Localization by Muscleblind Proteins

ABSTRACT: The Muscleblind-like (Mbnl) family of RNA-binding proteins plays important roles in muscle and eye development and in Myotonic Dystrophy (DM), where expanded CUG or CCUG repeats functionally deplete Mbnl proteins. We identified transcriptome-wide functional and biophysical targets of Mbnl proteins in brain, heart, muscle, and myoblasts using RNA sequencing and crosslinking/immunoprecipitation-sequencing approaches. This analysis identified several hundred splicing events whose regulation depended on Mbnl function, in a pattern indicative of functional interchangeability between Mbnl1 and Mbnl2. A nucleotide resolution RNA map associated repression or activation of exon splicing with Mbnl binding near either 3' splice site or near the downstream 5' splice site, respectively. Transcriptomic

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Eric Wang 

PROVIDER: E-GEOD-39911 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress