Exposure of microaerobic Campylobacter jejuni to diffusible molecules from chicken caecal contents

ABSTRACT: Two batch cultures of wild-type C. jejuni NCTC 11168 were grown in 200 ml volumes of Mueller-Hinton broth in 500 ml baffled flasks. Microaerophilic conditions were generated using a MACS-VA500 microaerophilic work station (10% Oxygen, 10% Carbon dioxide, 80% Nitrogen) from Don Whitley Scientific, Ltd, which also maintained the growth temperature at 42 M-BM-:C. When mid-exponential phase was reached, a custom-made diffusion capsule (as described in Pirt, 1971) containing chicken caecal contents was placed for 10, 30, or 60 minutes. After the exposure, samples of both treated and untreated cells were harvested into phenol/ethanol to stabilize the RNA and total RNA was purified using Qiagen's RNeasy Mini kit (as recommended by the suppliers) prior to use in microarray analysis. Batch cultures

ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

SUBMITTER: Robert Poole 

PROVIDER: E-GEOD-39917 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress