Knockdown of Hnrnpa0, a del(5q) Gene, Alters Myeloid Cell Fate in Murine Cells

ABSTRACT: To understand the effect of loss of Hnrnpa0, an RNA-binding protein shown to regulate transcript stability via binding to the AU-rich elements (AREs) of mRNAs, on myelopoiesis, we used RNAi interference to model Hnrnpa0 loss in an experimental murine cell system, and then used global expression profiling techniques to determine the impact of that knockdown on gene expression, especially ARE-containing genes. The PUER system is a murine myeloid progenitor cell line expressing the tamoxifen (4-OHT) responsive, PU.1-ER fusion gene, allowing controlled myeloid differentiation in cells in which Hnrnpa0 expression has been altered via RNAi technology. By examining perturbations in gene expression both before and during myeloid differentiation, we have shown the Hnrnpa0 loss preferentially alters

ORGANISM(S): Mus musculus

SUBMITTER: David Young 

PROVIDER: E-GEOD-39934 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress