Genome-wide Polycomb localization in Drosophila whole testes and purified germline precursor cells.

ABSTRACT: The Drosophila spermatogenesis cell differentiation pathway involves the activation of a large set of genes in primary spermatocytes. Most of these genes are activated by testis-specific TATA-binding protein associated factors (tTAFs). In the current model for the activation mechanism, Polycomb plays a key role silencing these genes in the germline precursors, and tTAF-dependent activation in primary spermatocytes involves the displacement of Polycomb from gene promoters. We investigated the genome-wide binding of Polycomb in wild type and tTAF mutant testes. According to the model we expected to see a clear enhancement in Polycomb binding at tTAF-dependent spermatogenesis genes in tTAF mutant testes. However, we find little evidence for such an enhancement in tTAF mutant testes compared

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sherif El-Sharnouby 

PROVIDER: E-GEOD-39935 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress