A human genome-wide screen for regulators of clathrin-coated vesicle formation

ABSTRACT: Clathrin-mediated endocytosis is essential for a wide range of cellular functions. We used a multi- step siRNA-based screening strategy to identify novel regulators of the first step of clathrin- mediated endocytosis, formation of clathrin-coated vesicles (CCVs) at the plasma membrane. A primary genome-wide screen identified 334 hits that caused accumulation of CCV cargo on the cell surface. A secondary screen identified 92 hits that inhibited cargo uptake and/or altered the morphology of clathrin-coated structures. The hits include components of four functional complexes: coat proteins, V-ATPase subunits, spliceosome-associated proteins, and acetyltransferase subunits. Electron microscopy revealed that V-ATPase depletion caused the cell to form aberrant non-constricted clathrin-coated str

ORGANISM(S): Homo sapiens

SUBMITTER: Patrycja Kozik 

PROVIDER: E-GEOD-39954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress