ChIP-seq analysis of SRT1 and H3K9ac in MH63 and srt1 RNAi.

ABSTRACT: We report the high-throughput profiling of H3K9ac and SRT1 in MH63 and SRT1 RNAi. By obtaining about 8 million pair-end reads from H3K9ac and 6 million pair-end reads from SRT1, we find that about 45% of rice genes are marked by H3K9ac in 11 day-old seedlings. There are 1824 genes may be bound directly by OsSRT1. OsSRT1 is a site-specific deacetylase involved in deacetylation of H3K9 over specific genes. Overlapping between increased H3K9ac peaks in the RNAi plants and that of OsSRT1-binding was found over 37 genes, suggesting that OsSRT1 directly targeted to these genes for H3K9 deacetylation. The observations that H3K9ac was enriched at the 5M-bM-^@M-^Y end of genes larger than 1.5 kb and OsSRT1 RNAi enhanced the enrichment at that position. However, the OsSRT1-binding was found to be en

ORGANISM(S): Oryza sativa

SUBMITTER: zhong xiaochao 

PROVIDER: E-GEOD-40054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress