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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Role of CAMTA1 gene under drought stress

ABSTRACT: The CAMTA1 mutant and Col-0 were studied under water and drought condition. The camta1 showed stunted primary root growth under osmotic stress. The expression analysis revealed drought recovery as major indicative pathway along with membrane and chloroplast related protein in camta1 under drought stress. Large number of positively regulated genes were related to osmotic balance, transporters, AP2 and ABA. We used Affymetrix expression analysis to validate the role of CAMTA1 under drought stress. The RNA of Col-0 and camta1 mutant were isolated in water and drought condition.The ATH1 gene chip was used for expression analysis.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: samir sawant

PROVIDER: E-GEOD-40061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CAMTA 1 regulates drought responses in Arabidopsis thaliana.

Pandey Neha N Ranjan Alok A Pant Poonam P Tripathi Rajiv K RK Ateek Farha F Pandey Haushilla P HP Patre Uday V UV Sawant Samir V SV

BMC genomics 20130402

<h4>Background</h4>Transcription factors (TF) play a crucial role in regulating gene expression and are fit to regulate diverse cellular processes by interacting with other proteins. A TF named calmodulin binding transcription activator (CAMTA) was identified in Arabidopsis thaliana (AtCAMTA1-6). To explore the role of CAMTA1 in drought response, the phenotypic differences and gene expression was studied between camta1 and Col-0 under drought condition.<h4>Results</h4>In camta1, root development ...[more]

PMID: 23547968

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Role of CAMTA1 gene under drought stress

Project description:The CAMTA1 mutant and Col-0 were studied under water and drought condition. The camta1 showed stunted primary root growth under osmotic stress. The expression analysis revealed drought recovery as major indicative pathway along with membrane and chloroplast related protein in camta1 under drought stress. Large number of positively regulated genes were related to osmotic balance, transporters, AP2 and ABA. We used Affymetrix expression analysis to validate the role of CAMTA1 under drought stress.

2012-08-13 | GSE40061 | GEO

Overexpression of AtCAMTA5 enhances drought tolerance in Arabidopsis.

Project description:The Overexpression of AtCAMTA5 leads to higher plant weight and seed weight as compare to Col-0. Under drought condition overexpression lines had reduce water loss,high water use efficiency decline in photosynthesis contributes to 95% survivability wherase Col-0 and camta5 showed enhance sensitivity.Microarray analysis revealed that AtCAMTA5 regulates genes enriched with CAMTA recognition motif like ANAC19, SPL16, aminotransferase, chitinase, MAPK7, SnRK2.2, E3-ligase, RUBISCO, PSI and PSII, etc which were involved in stress response, development, protein modification and photosynthesis. We used affymatrix expression analysis to validate role of CAMTA5 under drought stress along with physiological and biochemical assay.

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Genome-wide analysis of stress-responsive genes and alternative splice variants in Arabidopsis roots under osmotic stresses

Project description:The shoots and roots of a plant respond differently to osmotic stress, as they have distinct functions and anatomical structures. Under conditions of high solute concentration, such as in saline soils or drought, water uptake by the roots is reduced, resulting in cellular dehydration. In this study, we performed transcriptional profiling of roots of Arabidopsis under osmotic stress conditions such as high salinity and drought using mRNA-Seq for the assessment of gene expression changes in roots of Arabidopsis. mRNA-Seq analysis showed that many differentially expressed genes showed differential expressions under both salt stress and drought stress conditions in roots and were distinct from aerial parts. We confirmed 68 transcription factor genes which is involved in osmotic stress signal transduction in roots and are connected tightly. Interestingly, well-known ABA-dependent and/or -independent osmotic stress-responsive genes were less increased in roots, indicating that osmotic stress response in roots might be regulated by stress pathways other than well-known pathways. We identified 26 osmotic stress-responsive genes, which have alternative splicing variant isoforms, showed distinct expression in roots under osmotic stress conditions from the mRNA-Seq analysis. Quantitative RT-PCR confirmed that alternative splicing variants, such as ANNAT4, MAGL6, TRM19, and CAD9, have differential expressions in roots under osmotic stress conditions, indicating that alternative splicing is an important regulatory mechanism in osmotic stress response in roots. Taken together, our study suggest that many transcription factor families are involved in osmotic stress response in roots and tightly connected each other. In addition, alternative splicing and function of alternative splicing variant isoforms are also important in osmotic stress response in roots. To understand the alternative splicing mechanism in roots, further study is necessary.

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Expression data in an Arabidopsis cytokinin (CK)-signaling mutant, ahp2,3,5 and its wild type plant (Col-0) under drought and well-water (control) conditions.

Project description:To understand the role of CK-signaling components, AHPs (His-containing phosphotransfer proteins) in drought stress response, we have employed transcriptional profiling of ahp2,3,5 and it's wild type plant, Col-0 under drought and well-water (control) conditions. Aligent’s Whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.

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ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells

Project description:In plants, drought stress is a major growth limiting factor causing cell water loss through open stomata. In this study, guard cell-specific transcripts from drought-stressed Arabidopsis plants were analyzed and a down-regulation of β-amylase 1 (BAM1) was found. In previous studies, BAM1 was shown to be involved in stomatal starch degradation under ambient conditions. Impaired starch breakdown of bam1 mutant plants was accompanied by decreased stomatal opening. Here, we show that drought tolerance of bam1 mutant plants is improved as compared to wild type controls. Microarray-analysis of stomata-specific transcripts from bam1 mutant plants revealed a significant down-regulation of genes encoding aquaporins, auxin- and ethylene-responsive factors and cell-wall modifying enzymes. This expression pattern suggests that reduced water-uptake and limited cell wall extension are associated with the closed state of stomata of bam1 mutant plants. Together these data suggest that regulation of stomata-specific starch turnover is important for adapting stomata opening to environmental needs and its breeding manipulation may result in drought tolerant crop plants. Stress induced gene expression in Arabidopsis leaves and Stomata was measured after exposure to single drought stress. Drought stress conditions were analysed for both, Col-0 plants and a T-DNA insertion line for β-amylase 1. Six week old plants were treated with drought stress (5 days) according to Prasch and Sonnewald, 2013. Two to three biological replicates have been hybridized for each treatment.

2015-06-30 | E-GEOD-59315 | biostudies-arrayexpress

Early drought-responsive changes in pepper leaves and roots

Project description:Drought is one of the major factor that limits crop production and reduces yield. To understand the early response of plants under nearly natural conditions, pepper plants were grown in a greenhouse and drought stressed by withholding water for one week. Plants adapted to the decreasing water content of the substrate by adjustment of their osmotic potential in roots by accumulation of raffinose, glucose, galactinol and proline. In contrast in leaves levels of fructose, sucrose and also galactinol increased. Due to the water deficit cadaverine, putrescine, spermidine and spermine accumulated in leaves whereas the concentration of polyamines was reduced in roots. These polyamines are suggested to rather act as stress protectants than for osmotic adjustment. To understand the molecular basis of the response to this early drought stress better, four suppression subtractive hybridisation libraries from leaves and roots were constructed. Microarray technique was used to identify differentially expressed genes. A total of 109 unique ESTs were detected. The diversity of the putative functions of all identified genes confirms the complexity of the plant response to drought stress. Keywords: Transcription profiling

2009-10-28 | GSE18627 | GEO

Transcription profiling of Arabidopsis srk2cf mutant plants under drought stress for 0, 1 and 4 hours

Project description:Microarray experiments were performed using Arabidopsis wild type plants (Col-0) and srk2cf double knockout mutants to investigate functions of two osmotic stress-activated protein kinases, SRK2C and SRK2F. Transcription profiles of wild type and mutants were compared under drought stress for 0, 1 and 4 h.

2009-10-15 | E-MEXP-2377 | biostudies-arrayexpress

Responses of Arabidopsis leaves to prolonged osmotic stress are mediated by their developmental stage

Project description:Drought is an important environmental factor affecting plant growth and biomass production. Despite this importance, little is known on the molecular mechanisms regulating plant growth under water limiting conditions. The main goal of this work was to investigate, using a combination of growth and molecular profiling techniques, how Arabidopsis thaliana leaves adapt their growth to prolonged mild osmotic stress. Fully proliferating, expanding and mature leaves were harvested from plants grown on plates without (control) or with 25mM mannitol (osmotic stress) and compared to seedlings at stage 1.03. Total RNAs were extracted using Trizol method from vegetative part of seedlings at stage 1.03 and leaves that are fully proliferating, expanding or mature. All plants were grown in vitro on medium without (0mM) or with mannitol (25mM) in three independent biological experiments. Each sample was pooled from multiple plants and multiple plates in one experiment. RNA samples were submitted to ATH1 array hybridization.

2010-04-11 | E-GEOD-16474 | biostudies-arrayexpress

Role of NPR1 in SA mediated transcription

Project description:Affymetrix expression analysis was used to validate the role of NPR1 in SA mediated transcription. The RNA of col-0 and npr1-1 mutant were isolated after 24hr water and SA treatment.The ATH1 gene chip was used for expression analysis.

2013-12-24 | E-GEOD-51626 | biostudies-arrayexpress

ß-amylase1 mutant Arabidopsis plants show improved drought tolerance due to reduced starch breakdown in guard cells

Project description:In plants, drought stress is a major growth limiting factor causing cell water loss through open stomata. In this study, guard cell-specific transcripts from drought-stressed Arabidopsis plants were analyzed and a down-regulation of β-amylase 1 (BAM1) was found. In previous studies, BAM1 was shown to be involved in stomatal starch degradation under ambient conditions. Impaired starch breakdown of bam1 mutant plants was accompanied by decreased stomatal opening. Here, we show that drought tolerance of bam1 mutant plants is improved as compared to wild type controls. Microarray-analysis of stomata-specific transcripts from bam1 mutant plants revealed a significant down-regulation of genes encoding aquaporins, auxin- and ethylene-responsive factors and cell-wall modifying enzymes. This expression pattern suggests that reduced water-uptake and limited cell wall extension are associated with the closed state of stomata of bam1 mutant plants. Together these data suggest that regulation of stomata-specific starch turnover is important for adapting stomata opening to environmental needs and its breeding manipulation may result in drought tolerant crop plants. Stress induced gene expression in Arabidopsis stomata was measured after exposure to single heat stress. Heat stress conditions were analyzed for both Col-0 plants and a T-DNA insertion line for β-amylase 1. Three days before harvesting heat stress was applied (32°C/28°C). Samples were taken by pooling the stomata of six to eight leaves per sample.

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