Molecular targets of doxazosin in human prostatic stromal cells

ABSTRACT: BACKGROUND: We used cDNA microarray analysis to obtain insights into the mechanisms of action of doxazosin, an alpha(1)-adrenergic receptor antagonist used to treat benign prostatic hyperplasia (BPH). METHODS: Hierarchical clustering analysis and significance analysis of microarray (SAM) were performed to identify genes differentially expressed between untreated stromal cells cultured from normal tissue and BPH, and changes in gene expression induced by doxazosin. Transcript levels of selected genes were validated by real-time reverse-transcription polymerase chain reaction (RT-PCR). : Hierarchical clustering analyses separated untreated normal and BPH cells. Sixty-seven genes whose expression varied at least twofold after doxazosin treatment in both normal and BPH cells were identified, a

ORGANISM(S): Homo sapiens

SUBMITTER: Hongjuan Zhao 

PROVIDER: E-GEOD-4010 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress