Genome-wide distribution of 5-formylcytosine in ES cells is associated with transcription and depends on TDG

ABSTRACT: Methylation of cytosine in DNA (5mC) is an important epigenetic mark that is involved in the regulation of genome function. During early embryonic development in mammals, the DNA methylation landscape is dynamically reprogrammed in part through active demethylation. Recent advances have identified key players involved in active demethylation pathways, including oxidation of 5mC to 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) by the TET family of enzymes and excision of 5fC by the base excision repair enzyme thymine DNA glycosylase (TDG). Here, we provide the first genome-wide distribution map of 5fC in mouse embryonic stem (ES) cells and evaluate potential roles for 5fC in differentiation. Our method exploits the unique reactivity of 5fC to link a biotin tag for pulldown and h

ORGANISM(S): Mus musculus

SUBMITTER: Dario Beraldi 

PROVIDER: E-GEOD-40148 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress