Transcription profiling of Arabidopsis leaves reponse to DTT to examine the effect of redox on gene expression

ABSTRACT: In this study, we used GC-MS analysis in combination with flux analysis and the Affymetrix ATH1 GeneChip to survey the metabolome and transcriptome of Arabidopsis leaves in response to manipulation of the thiol-disulfide status. Feeding low concentrations of the sulfhydryl reagent dithiothreitol (DTT) for one hour at the end of the dark period led to post-translational redox-activation of ADP-glucose pyrophosphorylase and major alterations in leaf carbon partitioning, including an increased flux into major respiratory pathways, starch- cell-wall-, and amino-acid synthesis and a reduced flux to sucrose. This was accompanied by a decrease in the levels of hexose-phosphates, while metabolites in the second half of the TCA cycle and various amino acids increased, indicating a stimulation of an

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Joost van Dongen 

PROVIDER: E-GEOD-4021 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress