Gene Expression Changes in a Tumor Xenograft by a Py-Im Polyamide

ABSTRACT: Gene regulation by DNA binding small molecules could have important therapeutic applications. This study reports the investigation of a DNA-binding pyrrole-imidazole polyamide targeted to bind the DNA sequence 5M-bM-^@M-^Y-WGGWWW-3M-bM-^@M-^Y with reference to its potency in a subcutaneous xenograft tumor model. The molecule is capable of trafficking to the tumor site following subcutaneous injection and modulates transcription of select genes in vivo. A FITC-labeled analogue of this polyamide can be detected in tumor-derived cells by confocal microscopy. RNA deep sequencing (RNA-seq) of tumor tissue allowed the identification of further affected genes, a representative panel of which were interrogated by qRT-PCR and correlated with cell culture expression levels. Xenografts. Grafting with

ORGANISM(S): Homo sapiens

SUBMITTER: Jevgenij Raskatov 

PROVIDER: E-GEOD-40218 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress