Deep Clonal Profiling of Formalin-Fixed Paraffin-Embedded Clinical Samples

ABSTRACT: Formalin-fixed paraffin-embedded (FFPE) tissues are a vast resource of annotated clinical samples. As such, they represent highly desirable and informative materials for the application of high-definition genomics for improved patient management and to advance the development of personalized therapeutics. However, a limitation of FFPE tissues is the variable quality of DNA extracted for analyses. Furthermore, admixtures of non-tumor and polyclonal neoplastic cell populations limit the number of biopsies that can be studied and make it difficult to define cancer genomes in patient samples. To exploit these valuable tissues, we applied flow cytometry-based methods to isolate pure populations of tumor cell nuclei from FFPE tissues and developed a methodology compatible with oligonucleotide ar

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Barrett 

PROVIDER: E-GEOD-40299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress