Gene expression in an EMT model using an immortalized bronchial epithelial cell line induced by TGF-M-NM-21

ABSTRACT: This study aimed to establish an epithelial-mesenchymal transition (EMT) model with an immortalized human bronchial epithelial cell line, M-BE, and to identify an EMT signature gene set. The TGF-M-NM-21-induced M-BE cells got spindle-shaped fibroblast-like morphology and lost the cell-cell contact, with down-regulated expression of epithelial marker E-cadherin and up-regulated expression of mesenchymal markers N-cadherin and Vimentin. Examined by microarray, there were 2628 genes identified as significant EMT-related, including 1490 up-regulated genes (FC > 2, fdr < 0.01) and 1138 down-regulated genes (FC < 0.5, fdr < 0.01) in TGF-M-NM-21-induced M-BE cells. M-BE cells were treated with human recombinant TGF-M-NM-21 (5ng/ml) for six days. M-BE cells cultured without TGF-M-NM-21 were consid

ORGANISM(S): Homo sapiens

SUBMITTER: Bangrong Cao 

PROVIDER: E-GEOD-40374 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress