Transcription profiling of Arabidopsis plants overexpressing SlHsfA3

ABSTRACT: In order to investigate the possible molecular mechanisms of SlHsfA3 function in plant growth and stress responses, we employed the RNA-seq approach to identify the genes with altered expression levels in the transgenic Arabidopsis overexpressing SlHsfA3. Processing of RNA samples on the Illumina HiSeq 2000 system yielded more than 24 million reads, each 100bp in length, encompassing 2.4Gb of sequence data for each sample which was then mapped to the reference genome. Quantitative analysis of RNA-seq data identified substantial variation in expression profiles among different genotypes, consistent with known functional differences. Transcript abundance obtained from RNA-seq data was indicated as RPKMs and therefore, Up- and down-regulated genes were determined by a greater than 2-fold chan

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: jingfu li 

PROVIDER: E-GEOD-40388 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress