Genome-wide HSF1 binding sites in control and heat shocked spermatocytes and hepatocytes

ABSTRACT: In somatic cells elevated temperature induces activation of the heat shock transcription factor 1 (HSF1) what leads to heat shock proteins synthesis and cytoprotection. However, in the male germ cells (spermatocytes) upon HSF1 activation, caspase-3 dependent apoptosis is induced and spermatogenic cells are actively eliminated. To find out molecular targets of HSF1 in all promoter regions, and to elucidate a mechanism of such diverse HSF1 activity we carried out genome-wide HSF1 binding analysis in control and heat-shocked cells, either spermatogenic or somatic. As model somatic cells we used hepatocytes that respond to hyperthermia in a classical way by induction of heat shock genes transcription. As spermatogenic cells we used a fraction of cells enriched with spermatocytes, which are the

ORGANISM(S): Mus musculus

SUBMITTER: Joanna Polanska 

PROVIDER: E-GEOD-40390 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress