A functional genomics approach identifies the GATA factor SERPENT as a tissue-specific partner of CLOCK/CYCLE in the Drosophila circadian clock

ABSTRACT: Broadly expressed transcriptions factors (TFs) control tissue-specific programs of gene expression through interactions with local TF networks. Prime examples are the circadian clock TFs CLOCK (CLK) and CYCLE (CYC or BMAL1): while they control a core transcriptional circuit throughout animal bodies, downstream clock target genes and circadian physiology are tissue-specific. Here, we use ChIP-seq to determine the regulatory targets of Drosophila CLK and CYC, which we epitope-tagged by homologous recombination. Both TFs have distinct binding sites in heads versus bodies, suggesting that they directly control tissue-specific downstream target genes. Analysis of these context-specific binding sites revealed distinct sequence motifs for putative clock partner factors, including a motif for the

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Anais Bardet 

PROVIDER: E-GEOD-40467 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress