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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide GTL1 binding sites in Arabidopsis thaliana

ABSTRACT: To understand how GTL1 regulates cell growth, we first identified its potential direct targets by the chromatin immunoprecipitation followed by the hybridization on an Affymetrix Arabidopsis Tiling 1.0R array (ChIP-chip). To enrich the genomic region bound by GTL1 in vivo, we harvested whole aerial parts of 12-day-old gtl1-1 plants complemented with the pGTL:GTL1:GFP constructs and immunoprecipitated the chromatin fragments associated with GTL1-GFP proteins using antibodies against GFP. After applying a cut-off P-values of 0.001of MAT (Model-based analysis of tiling array), we identified a total number of 3,900 putative immediate target genes that showed consistent binding by GTL1. Two IP chips compared to two Input chips.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kengo Morohashi

PROVIDER: E-GEOD-40519 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide GTL1 binding sites in Arabidopsis thaliana

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