Global spatial organization of M-NM-3 proteobacterial nucleoids is determined by specific long-range interactions (E. coli)

ABSTRACT: GCC was used to determine the structure of E. coli grown in LB or treated with SHX. The bacterial genome is highly condensed into a nucleoid structure. Here we present global analyses of the genome spatial organization for two M-NM-3-proteobacteria: Escherichia coli and Pseudomonas aeruginosa by Genome Conformation Capture. Long distance interactions occurred within the E. coli and P. aeruginosa nucleoids with frequencies that were affected by growth condition and gene dosage. Spatial clustering of genes that are either up or down-regulated depended on the environmental signals, indicating a non-random functional organization of the nucleoid. The largest changes in gene expression upon amino acid starvation occurred in genes that participate in long-range interactions. These genes remaine

ORGANISM(S): Escherichia coli

SUBMITTER: Justin O'Sullivan 

PROVIDER: E-GEOD-40603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress