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Effects of co-culture of Endothelial Cells with Mesenchymal Stem Cells on Gene Expression

ABSTRACT: Study designed to explore the effects of endothelial cell/MSC co-culture on individual gene expression profile of each cell type 4 independent samples from each of 4 groups: MSCs cultured alone; Pulmonary endothelial cells cultured alone; MSCs co-cultured with PECs then FACS separated; PECs co-cultured with MSCs then FACS separated

ORGANISM(S): Homo sapiens

SUBMITTER: LI SHEN

PROVIDER: E-GEOD-40613 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mesenchymal stem cells regulate blood-brain barrier integrity through TIMP3 release after traumatic brain injury.

Menge Tyler T Zhao Yuhai Y Zhao Jing J Wataha Kathryn K Gerber Michael M Zhang Jianhu J Letourneau Phillip P Redell John J Shen Li L Wang Jing J Peng Zhalong Z Xue Hasen H Kozar Rosemary R Cox Charles S CS Khakoo Aarif Y AY Holcomb John B JB Dash Pramod K PK Pati Shibani S

Science translational medicine 20121101 161

Mesenchymal stem cells (MSCs) may be useful for treating a variety of disease states associated with vascular instability including traumatic brain injury (TBI). A soluble factor, tissue inhibitor of matrix metalloproteinase-3 (TIMP3), produced by MSCs is shown to recapitulate the beneficial effects of MSCs on endothelial function and to ameliorate the effects of a compromised blood-brain barrier (BBB) due to TBI. Intravenous administration of recombinant TIMP3 inhibited BBB permeability caused ...[more]

PMID: 23175708

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