The Genomic Binding Profile of GAGA Element Associated Factor (GAF) in Drosophila S2 cells

ABSTRACT: GAGA associated transcription factor (GAF) is a highly abundant and essential protein in Drosophila. GAF recognizes and binds arrays of GA dinucleotides via a zinc finger DNA binding domain to regulate transcription by binding to general TF machinery or recruit nucleosome remodeling factors. We performed GAF ChIP-seq to quantify the intensity of GAF binding at high resolution in S2 cells. In addition, we performed GAF ChIP-seq in S2 cells that were depleted of GAF by RNAi. By quantifying the degree to which all GAF binding sites are susceptible to GAF depletion, we found the cellular degree of depletion does not translate equally to the depletion of GAF at individual chromatin bound sites. For example, some high intensity GAF binding sites were completely unaffected by GAF depletion, while

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Michael Guertin 

PROVIDER: E-GEOD-40646 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress